Antibacterial effects of the leaves and twigs of Turraea vogelii on some enteric pathogens

Haishat Y. Olufadi-Ahmed; Philip A. Idowu; Haruna J. Audu

doi:10.54117/gjpas.v2i1.95

Authors

Haishat Y. Olufadi-Ahmed Department of Pharmaceutical Microbiology & Biotechnology, University of Ilorin, Ilorin, Nigeria.
Philip A. Idowu Department of Pharmaceutical Microbiology, University of Ibadan, Ibadan, Nigeria.
Haruna J. Audu Department of Pharmaceutical Microbiology & Biotechnology, Kaduna State University, Kaduna, Nigeria.

DOI:

https://doi.org/10.54117/gjpas.v2i1.95

Keywords:

Turraea vogelii, Leaves, Twigs extract, Antibacterial, Enteric pathogens, Clinical isolates

Abstract

Qualitative and quantitative phytochemical analysis as well as antibacterial analysis of extracts of Turraea vogelii (Hook F.) leaves and twigs on some enteric pathogens were reported in the current study. The qualitative phytochemical screening of the leaf extracts showed the presence of saponins, flavonoids, cardiac glycosides, anthraquinones, terpenoids, steroids and alkaloids. In addition, the quantitative phytochemical screening showed that the greatest percentage yield was from the methanol extract with alkaloids (8.8%) and terpenoids (8.7%) showing the highest concentrations. The antibacterial effects of hexane, ethyl acetate and methanol extracts of Turraea vogelii at concentrations 80 mg/mL, 40 mg/mL and 20 mg/mL each were studied by the pour plate method. Clinical isolates of Escherichia coli, Salmonella typhi and Proteus mirabilis (five each) obtained from University College of Health (UCH), Ibadan, Nigeria were employed as test organisms. Gentamicin was used as control at concentration of 10 µg/mL. Preliminary antimicrobial assay using only methanol showed antimicrobial activity in both leaves and twigs. Results showed that Escherichia coli was most susceptible to all extracts of the leaves while Proteus mirabilis was the least susceptible. For the twigs, Salmonella typhi was most susceptible to the hexane extract, E. coli was most susceptible to ethyl acetate extract and Proteus mirabilis was most susceptible to the methanol extract. Minimum Inhibitory Concentration (MIC) of leaves showed that its hexane extract is bacteriostatic at 40 mg/mL but at 20 mg/mL, it showed bacteriostatic activity against Proteus mirabilis. The methanol extract of the twigs had an MIC of 20 mg/mL for most of the isolates collected except the Salmonella typhi that had an MIC of 40 mg/mL. Minimum Bactericidal Concentration (MBC) of the ethyl acetate extract of leaves and methanol extract of twigs was 40 mg/mL. This study has demonstrated the antibacterial effect of leaves and twigs extracts of T. vogelii on some enteric pathogens.

References

Adam Mustapha, Mustafa Alhaji Isa, Tijani Isa, Ibrahim Yusuf Ngoshe and Hashidu Bala (2020). Detection of Multidrug Resistant Gram Negative Bacteria in Healthy Cattle from Maiduguri Metropolitan, Nigeria. Journal of Advances in Microbiology 20(6): 68-76.

Akinyemi, K.O., Oladapo, O., Okwara, C.E., Ibe, C.C. and Fasure, K.A. (2005). Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC Complimentary Alternative medicine, 5:6.

Alfalluos, K. A. (2017). Qualitative and quantitative phytochemical analysis and antimicrobial activity of “Retama” extract grown in Zliten Libya. International Journal of Medical Science and Clinical Invention, 4(4). Ijmsci. 4(4): 2861-2866

Andrews, J.M. (2001). Determination of Minimum Inhibitory Concentrations. Journal of Antimicrobial Chemotherapy 48:5-6.

Bonjar, G.H., Nik, A.K. and Aghighi, S. (2004). Antibacterial and antifungal survey in plants used in indigenous herbal-medicine of south east regions of Iran. J Biol Sci. 4:405–12.

Burkhill, H.M. (1985). The useful plants of West Tropical Africa 2nd edition volume 1. Royal botanical garden, Kew bulletin 389-391

Clinical and Laboratory Standards Institute (2014). Development of in vitro Susceptibility testing criteria and Quality control parameters; Approved Guideline – Third Edition.

Cowan, M.M. (1999). Plant products as antimicrobial agents. Clin Microbiol Rev. 12:564–82.

Elgayyar M., Draughon F.A, Golden D.A., Mounth J.R. (2001). Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. J Food Prot. Volume 64

Fabry, W., Okemo, P.O. and Ansorg, R. (1998). Antibacterial activity of East African medicinal plants. J Ethnopharmacol. 60:79-84.

Geidam, Y.A., Ambali, A.G. and Onyeyli P.A. (2007). Preliminary phytochemical and antibacterial evaluation of crude aqueous extracts of Psidium guajava L. leaf. J Appl Sci 7:511-4.

Griffin, S.G., Wyllie S.G., Markham J.L., Leach D.N. The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour Fragr. J. 1999; 14:322–332.

Hidayah, Ayodeji Olumoh-Abdul, Rashidat Oluwafunke Ayanniyi, Fatimoh Idowu Ojuade, Remilekun Justina (2019). Evaluation of nutraceutical content and topical Anti-inflammatory activities of Turraea vogelii Hook F. Ex. Benth (Meliciaea). ijt.ir 13(1); 35-39.

Joseph, A.A., Odimayo, M.S., Olokoba, L.B., Olokaba, A.B. and Popoola GO (2017). Multiple antibiotic resistance index of Escherichia coli isolates in a Tertiary Hospital in SouthWest Nigeria. Medical Journal of Zambia. 44(4):225-232.

Lee, J.H., Park, K.H., Lee MH, Kim HT, Seo WD, Kim WD (2013). Identification, characterization, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chem 136:843-53.

Odugbemi T. (2008). A textbook of Medicinal plants in Nigeria. Tolu Press Lagos 23-97.

Ogbole, O.O., Saka Y.A., Fasinu, P.S., Fadare, A.A, Ajaiyeoba E.O (2016). Antimalarial and cytotoxic properties of Chukrasia tabularis A. Juss and Turraea vogelii Hook F. Ex. Benth. Parasitol Res. 115(4): 1667-1674.

Olufadi-Ahmed, H.Y. and Idowu, P.A, (2019). Preliminary phytochemical screening and Antioxidant Property of Leaf and twig Extracts of Turraea Vogelii Hook. f. ex. Benth (Meliaceae). J. Pharm. Res. Dev. & Pract., April, 2019, Vol. 3 No. 1, P 1-8

Pandey, A. K., and Kumar, S. (2013). Perspective on plant products as antimicrobial agents: a review. Pharmacologia 4, 469–480.

Peng, H. and Mabberley, D.J. (2008). Flora of China, vol. 11. Beijing: Science press; 117-8.

Sahreen, S., Khan, M.R., Khan, R.A (2010). Evaluation of antioxidant activities of various extracts of Carissa opaca fruits. Food Chem, Volume 122 Issue 4 pp. 1205-1211.

Sharma, B.L, Daulat Singh, Santosh Sharma K, Afzal Hashmi, Arjun Singh, Anil Bansal (2013). Studies on some primary metabolite’s extraction and Quantification in different plant parts of selected Cassia Species. Asian J Pharm Clin Res 6:309-14.

Trease, G.E. and Evans, W.C. (1989). Pharmacognosy, Brailliar Tiridel Can, 13th ed. Macmillan Publishers.

Turnidge, J.D., Ferraro, M.J. and Jorgensen, J.H. (2003). Susceptibility test methods: General considerations. In Murray P.R., Baron E.J., Jorgensen J.H., Pfaller M.A., Yolken R.H. Manual of clinical Microbiology. 8thEd.Washington. American Society of Clinical Microbiology. 1103.

Van Rensburg S.J., Daniels W.M., Van Zyl J.M., and Taljaard, J.J. (2000). A comparative study of the effects of cholesterol, beta-sitosterol, beta-sitosterol glucoside, dehydroepiandrosterone sulphate and melantonin on invitro lipid peroxidation. Metab brain Dis. 15; 257-265

Walkty, A., Adam, H., Baxter, M., Denisuik, A., Lagace-Wiens P., Karlowsky, J.A., et al. (2014). In vitro activity of plazomicin against 5015 Gram-negative and Gram-positive clinical isolates obtained from patients in Canadian hospitals as part of the CANWARD study 2011-2012. Antimicrob Agents Chemother,58;5 2554-2563.

Zablotowicz, R.M., Hoagland R.E., Wagner, S.C. (1996). Effect of saponins on the growth and activity of rhizosphere bacteria. Adv Exp Med Biol. 405:83–95.